Performing genetic testing typically requires a lab and a scientist with molecular knowledge. This process typically requires PCR (polymerase chain reaction) which requires purified DNA extracted from your plant material, a sterile lab to limit contamination, and a precise machine capable of increasing and decreasing temperatures in a fine-tuned manner to allow for amplifying a molecular address to identify a particular trait. Using this process requires an investment of ~$40,000 plus the necessary skills to run the machine that typically requires years to master. Additionally, in order to read the results of this test requires several different, potentially harmful chemicals, in addition to the ability to interpret what the results mean.
i-Test simplifies this process by allowing for the extraction of DNA or RNA and amplification in a single process, incubating at a large temperature range thus allowing for common household items to be used, and an easy readout through a color change. We developed a brand new type of isothermal amplification that we term LARIAT.
The science behind this type of testing is anything but simple. We have done the hard work to simplify the process. Identifying the molecular address and pattern that correlates the genotype and phenotype, manufacturing the reaction to achieve consistent results, and quality controlling the genetic variability are processes that all take time, expertise, and resources.